New study finds seed transmission of TYLCV

[alexita]

The site's search function doesn't work for me (I'm on mobile), so I hope I'm not double-posting this.

As if Tomato Yellow Leaf Curl Virus weren't bad enough, this recent study published in Nature found it can be transmitted through seeds. Their studies were conducted at two Korean greenhouse farms, and in a growth chamber at a Korean university where they tested a TYLVC-tolerant cultivar against a susceptible cultivar.

So, yeah. World's over? Someone come tell me why their methods stink and these results mean nothing.

[ginger2778]

Originally Posted by alexita

The site's search function doesn't work for me (I'm on mobile), so I hope I'm not double-posting this.

As if Tomato Yellow Leaf Curl Virus weren't bad enough, this recent study published in Nature found it can be transmitted through seeds. Their studies were conducted at two Korean greenhouse farms, and in a growth chamber at a Korean university where they tested a TYLVC-tolerant cultivar against a susceptible cultivar.

So, yeah. World's over? Someone come tell me why their methods stink and these results mean nothing.

Oh $hit!!!!

[alexita]

I was looking for whitefly mesh online when I found it. Totally messes up my plans!

[kurt]

I had my dance with the virus Oct.2015.I went into quarantine Nov.Dec 2015.Got rid of everything from that batch including whiteflys with the crud.Started fresh seed,mix, nuked containers etc.Now I have 6-7 footers bursting with blossoms.Having a nice little cool run down south here and all plants are alive and kicking.

[ginger2778]

Somebody pointed out it might just be on the coat, not in the embryo inside. The article doesn't specify. Fermentation should fix it if outside on the seed coat.

[alexita]

They state the seeds grown in the farm greenhouses were from fruit that was left to drop, which I imagine must go through a natural fermentation.

The seed grown at the university grow house were removed from the fruit, washed in distilled water, soaked in 70% ethanol for 10 mins. and then 10% Clorox for 20 mins. I can't imagine the virus not being inactivated and denatured if it were only on the seed coat. Then the embryos from unplanted seeds were carefully removed from the seed coat in sterile conditions and tested for TYLCV, which they tested positive for.

All I see is doom and gloom! Or is it gloom and doom?

[carolyn137]

All viruses and viroids tested to date, as far as I know,have been found inside the seed,so surface decontamination/fermentation,etc.won't help.

For stuff inside the seed you have to use hot water treatment which is not easy for a home grower to do,and I'm sure you've seen in commercial catalogs the option of getting seeds for X that are or are not hot water treated.

And I'd fetch some links from the search function here if I had the time,but I don't,just enter hot water seed treatment,or similar and look for posts first,instead of threads.

Carolyn

[alexita]

Kurt, you only dealt with TYLCV once? That's really surprising and reassuring to me, considering you're in Homestead. You got any tricks you could share?

When I grew tomatoes in Kendall I had constant problems with it. I wasn't even close to agricultural areas. I could get creative to avoid whitefly for a month or two but most my plants succumbed to it. I only use bio control and mechanical pesticides though - I guess that's asking for it.

[ginger2778]

Originally Posted by alexita

They state the seeds grown in the farm greenhouses were from fruit that was left to drop, which I imagine must go through a natural fermentation.


Yes, I thought of that too. I was just hoping.


The seed grown at the university grow house were removed from the fruit, washed in distilled water, soaked in 70% ethanol for 10 mins. and then 10% Clorox for 20 mins. I can't imagine the virus not being inactivated and denatured if it were only on the seed coat. Then the embryos from unplanted seeds were carefully removed from the seed coat in sterile conditions and tested for TYLCV, which they tested positive for.

Of course that treatment would denature most viruses. I missed that part of the article. Really doesn't look good. I think I might have to buy a sous vide for heat treating the seeds.


All I see is doom and gloom! Or is it gloom and doom?

[ginger2778]

I still love yellow sticky traps. They work so well. The whiteflies are strongly attracted to the yellow, and they last a whole season. It's the only thing that works for me. I just dont have whitefly isdues anymore, and hardly any leafminers either.

The little ones are whiteflies, the others are leafminer flies and other types of flies.

[kurt]

Originally Posted by alexita

Kurt, you only dealt with TYLCV once? That's really surprising and reassuring to me, considering you're in Homestead. You got any tricks you could share?

When I grew tomatoes in Kendall I had constant problems with it. I wasn't even close to agricultural areas. I could get creative to avoid whitefly for a month or two but most my plants succumbed to it. I only use bio control and mechanical pesticides though - I guess that's asking for it.

Pool enclosure controls the majority of insects."Outside"with all the fruit trees and assorted growths I do replenish a constant supply of green lacewings,mantis,parasitic wasps,ladybugs.In our area it seems the infestation of whiteflys has diminished a lot.I hear the county is releasing parasitic wasps,plus all the other cultural practices seems to help.I use the sticky traps "inside"for the maters and peppers.

[alexita]

So I guess there's still hope left in this world. If hot water treated seed should work, that's at least one way of ensuring a disease-free start.

Kurt, is your pool enclosure made with typical porch screen? I have a few rolls lying around but figured I needed something finer for whitefly.

[ginger2778]

My screen is the standard stuff, and whiteflies still get through.

Alexita, what are you going to get or use to keep them at 50C for 25 minutes without fluctuation in the temp? And it has to be wet treatment from what I read, so an oven or dehydrator won't do.
I think I am finally going to spring for that Sous Vide machine. I hear steaks come out awesome too!

[alexita]

Originally Posted by ginger2778

My screen is the standard stuff, and whiteflies still get through.

Alexits, what are you going to get or use to keep them at 50C for 25 minutes without fluctuation in the temp? And it has to be wet treatment from what I read, so an oven or dehydrator won't do.
I think I am finally going to spring for that Sous Vide machine. I hear steaks come out awesome too!

I haven't crossed that bridge yet!

[kurt]

After Andrew we switched to darker fiberglass pet grade material(smaller hole,more thread/SQ inch)we did it for mangoe and leaf/branch falling puncture protection,anti UV penetration.Never occurred to me whitefly size since at that time then we never had a whitefly problem.I have seen them land on screen but never crawl through.