Forum area for discussing hybridizing tomatoes in technical terms and information pertinent to trait/variety specific long-term (1+ years) growout projects.
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#16 |
Crosstalk™ Forum Moderator
Join Date: Aug 2006
Location: 8407 18th Ave West 7-203 Everett, Washington 98204
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Since I am now at, or near, the location of my daughter's wedding, I have a few moments during the next few days to contact the Rick Tomato folks at Davis, California. I will ask if anyone has a handle on bi-colored breeding genes relating to dominance, etc.
I may look at their gene bank for details of which genes might be available that would make a good test for breeding. I am not around my breeding notes now, so I will have to wing it with them. Tom Wagner |
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#17 |
Tomatovillian™
Join Date: Aug 2007
Location: Michigan's Sunrise Side
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Thanks, Tom!
john
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I know where food comes from... |
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#18 | ||
Crosstalk™ Forum Moderator
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I just off the phone will Roger Cheletat of the Tomato Resources Center here in Davis, California where I am staying at the moment. Unfortunately, he could only take enough time to return my call as he and his associates are busy with field trip preparations.
We talked in some detail about gene expression of (at), a recessive gene called (Apricot) which is noted for having yellow-pink flesh color. We also talked about gf, gs, and gr. Apparently since they are curators of genes rather than breeders, the information we are seeking is not known offhand by Roger. It also seems that some of the genes I have been using have mutated since the phenotypes are not as the descriptors of those genes delineate. Bi-colored fruits have been studied in the past, Roger states, but I will have to explore the database more completely before I talk with him again. The high pigment genes are bouncing around in many of my creations, but I am too rusty right now to explain how I am using the enhanced expression for flesh colors. To give you some idea of the complexity of the subject see these links below. Quote:
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Tom Wagner |
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#19 |
Buffalo-Niagara Tomato TasteFest™ Co-Founder
Join Date: Aug 2006
Location: The Niagara Frontier
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Couple months later.... anything else to add to this subject? Tom?
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#20 |
Tomatovillian™
Join Date: Nov 2008
Location: Minnesota
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It appears that hp-1 and hp-2 are mutations in two different transcription factors that each control expression of various light regulated genes. Transcription factors are regulatory genes often controlling a cascade of related genes in one or more biochemical pathways. The "high pigment" naturally occuring mutants hp-1 and hp-2 (and their various alleles) are characterized by an exagerated light responsiveness, darker green foliage, and increased fruit pigmentation. The increased fruit pigmentation is the result of increased accumulation or carotenoid pigments (primarily lycopene). Interestingly there is also an inceased level of production and accumulation of various other beneficial phytochemicals, including vitamins C and E. This led Levin et. al. (2003) to state that these characteristics make one or more of these hp genes/alleles attractive candidates for non-GE "functional" tomatoes. The term "functional foods" is now being used to describe foods with enhanced nutritional quality. A recent paper in Nature stirred some controversy reporting the production of GE tomatoes with transcription factors from Snapdragon that up-regulated anthocyanin production. Anthocyanin and carotene are common plant pigments, both now associated with various potential health benefits.
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#21 |
Tomatovillian™
Join Date: Dec 2007
Location: Espanola, New Mexico
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Good discussion. hp also appears to be associated with very firm fruit which is slow to ripen. I'll have to look up that article in Nature.
frogsleap - I plan to try backcrossing to L. hirsutum. Have to see about plant vigor and seed viability, but I think some of the desirable traits might appear - pest and disease resistance. However, increased sucrose levels may be single gene recessive. Like to incorporate that, though. Last edited by goodwin; December 28, 2008 at 04:35 PM. Reason: clarity |
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#22 | |
Cross Hemisphere Dwarf Project™ Moderator
Join Date: Jan 2006
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Patrina
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Truth is colourful, not just black and white. PP: 2005 |
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#23 |
Buffalo-Niagara Tomato TasteFest™ Co-Founder
Join Date: Aug 2006
Location: The Niagara Frontier
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Mentor Tom, where are you? Any updates?
Carolyn, could you please e-mail me the 2004 heart/paste list? By the way, I only got to grow 3 plants of the "KB Heart" cross and got this: one RL red one PL red one RL PINK! Any ideas on why these combos, plese feel free to discuss. I am growing/pursuing the RL Pink & PL red this season. Mark |
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#24 |
Buffalo-Niagara Tomato TasteFest™ Co-Founder
Join Date: Aug 2006
Location: The Niagara Frontier
Posts: 942
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One BIG bump... still lots of questions unanswered...
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#25 |
Crosstalk™ Forum Moderator
Join Date: Aug 2006
Location: 8407 18th Ave West 7-203 Everett, Washington 98204
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I was surprised to see how little I remember of what is still languishing on these threads. Sorry for being distracted.
I am smack dab in the middle of seed extraction and have little time to respond. I have several bulk progenies to photograph and describe. The genetics of selfing Glacier X Green Zebra is giving me too much to handle with all of the funny, but expected recombs happening. I have had a lot of potato leaf, reg leaf F-2's that were used in furthering the diversity of tomatoes of hundreds of crosses. Just writing that down on seed papers is about all I can do now. Maybe I will find some down time and post on these subjects. |
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#26 | |
Tomatovillian™
Join Date: Feb 2010
Location: USA
Posts: 94
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#27 |
Tomatovillian™
Join Date: Feb 2008
Location: Mid-Ohio
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Here is a little something I put together for a cross I am working with. I left out the fact that there is also dwarf/determinant/indeterminant factors going on.
http://tomatoville.com/showthread.php?t=11700 |
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#28 |
Tomatovillian™
Join Date: Apr 2009
Location: western North Carolina
Posts: 84
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The original question in this post may have already been answered and I may have overlooked it. In the gene lists, dominant genes are designated by a capital letter for the first letter of the abbreviation for the gene and recessive genes are designated by a lower case letter for the first letter in the abbreviation. Good examples of dominant genes are the disease resistance genes such as Ve, I, I-2, I-3, Mi, Sw-5, Tm-2. Good examples of recessive genes are sp, t, y, j-2. I hope this helps.
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#29 |
Buffalo-Niagara Tomato TasteFest™ Co-Founder
Join Date: Aug 2006
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Tom, are you still with us???
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#30 | ||
Crosstalk™ Forum Moderator
Join Date: Aug 2006
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Mark asks where I am several times a year it seems...the latest ...
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There are just too many tomato varieties out there and the interest in new lines from me would be rather redundant! I released quite a few tomato varieties and crosses in Europe last Fall but the wind went out of my sails when I came home to the states where we are over-run with varieties....and I lost some of my push to talk about tomatoes. Not to say I don't still work with the breeding and selection.....but I had to start with new cooperators this year as last year's cooperators were overwhelmed with too many varieties. I am struggling to find the time to transplant tomatoes in the greenhouse and work for a living full time. I try to transplant 50 to 72 varieties a day, and most of the time those varieties/crosses/segregates number from just a few to several hundred per line. On the potato sub forum I think I may have talked about potato seedling transplants as in my 502 hybrid plants of Skagit Valley Golds x bulk diploids for example. I thought about putting together a web site about my tomatoes but I failed for the umpteenth time to get the follow through to put a site even to a single page. I have thousands of pictures I took in Europe...many of my tomatoes grown there but I get this kind of message when I try to upload picture 5412... Quote:
Also...who would care to read about a cross of a pink and green striped crossed to a hybrid of Blue P-20 and Woolly Green Zebra...picking out the seedlings to replant that are showing woolliness, blue stems, and hoping for a 50% chance of those having vivid stripes? Who would want to know what I am looking for in that one in the F-2 generation? I have to worry if I can find homes for them locally for seed extraction and breeding purposes. |
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