Tomatoville® Gardening Forums


Notices

Forum area for discussing hybridizing tomatoes in technical terms and information pertinent to trait/variety specific long-term (1+ years) growout projects.

Reply
 
Thread Tools Display Modes
Old March 31, 2014   #16
Darren Abbey
Tomatovillian™
 
Darren Abbey's Avatar
 
Join Date: Jun 2012
Location: Minnesota
Posts: 586
Default

Quote:
Originally Posted by bower View Post
'Del', 'Beta', 'at', 'sh'.
Have you heard of any generally available varieties with these mutations?

So far, I've only got "Caro-rich" in my list as having 'Beta'/'B'. I've read about the others, but haven't found any variety names.
Darren Abbey is offline   Reply With Quote
Old March 31, 2014   #17
bower
Tomatovillian™
 
bower's Avatar
 
Join Date: Feb 2012
Location: Newfoundland, Canada
Posts: 6,794
Default

Quote:
Originally Posted by Darren Abbey View Post
Have you heard of any generally available varieties with these mutations?

So far, I've only got "Caro-rich" in my list as having 'Beta'/'B'. I've read about the others, but haven't found any variety names.

Another Beta variety called "Caro-Red" is named in this research on the competitive interaction of Del and Beta:
http://www.genetics.org/content/56/2/227.full.pdf
bower is offline   Reply With Quote
Old March 31, 2014   #18
Minnesota Mato
Tomatovillian™
 
Join Date: Aug 2011
Location: minnesota
Posts: 175
Default

here is a nice variety from victory seeds with high Beta/B. http://www.victoryseeds.com/tomato_podorok-fei.html
Minnesota Mato is offline   Reply With Quote
Old April 1, 2014   #19
bower
Tomatovillian™
 
bower's Avatar
 
Join Date: Feb 2012
Location: Newfoundland, Canada
Posts: 6,794
Default

A practical way to distinguish Beta orange from tangerine or apricot is that the Beta F1 cross to red or yellow fruit will be orange red, while the F1 cross of tangerine or apricot will be red.
For Beta vs tangerine, the segregating yellow cross rr should be easy to tell apart, since Beta is completely masked by rr ie the fruit is yellow, while yellow- tangerine rrtt is a pale orange color.
I guess I'll be watching for the colour of my Zolotoe Serdtse F1's, especially with Beta turning up in another Russian variety.
bower is offline   Reply With Quote
Old April 1, 2014   #20
crmauch
Tomatovillian™
 
crmauch's Avatar
 
Join Date: May 2013
Location: Honey Brook, PA Zone 6b
Posts: 399
Default

Quote:
Originally Posted by Darren Abbey View Post
Have you heard of any generally available varieties with these mutations?

So far, I've only got "Caro-rich" in my list as having 'Beta'/'B'. I've read about the others, but haven't found any variety names.
Jaune Flammee also has a Beta gene and is high in Beta Carotene. It appears there is variation in the Beta gene itself? (see next paragraph)

There is this presentation that doesn't have much text. It appears to being doing analysis on the Beta gene (or a promoter of the B gene?), and looking at breeding and using some sort of marker for improving selection, but given the lack of text, I'm only making inferences: http://tgc.ifas.ufl.edu/2013/2013/02%20-%20Orchard.pdf

I have some 97L97 seeds that I got from Fusion_Power. Mine have not yet come up, so I don't know yet if I'll have enough seed to share (If they don't come up this week, I'm going to restart them.) 97L97 was a 'breeding' tomato release from the USDA if my memory serves. It still has the Beta gene tied to the determinate characteristic.

Has anyone have information on how Delta-carotene reacts in the body? Looking at some synthesis pathways I've seen recently delta is a precursor to alpha-carotene which is 1/2 as effective as beta-carotene (inferred from some reading), . However the synthesis pathways I saw I think were detailing what happens in the plant.
crmauch is offline   Reply With Quote
Old April 1, 2014   #21
Darren Abbey
Tomatovillian™
 
Darren Abbey's Avatar
 
Join Date: Jun 2012
Location: Minnesota
Posts: 586
Default

I've got a figure I've put together about how the carotenoids are synthesized in tomatoes, but how we animals utilize the compounds is an entirely different subject.

Part of why I was looking for variety names was for a blog post talking about the carotenoid pathway mutants : http://the-biologist-is-in.blogspot....-tomatoes.html

The list of names is more comprehensive than is used in that post, however. I may do a later post more focussed on the names of varieties with certain genes.

Last edited by Darren Abbey; April 1, 2014 at 06:05 PM.
Darren Abbey is offline   Reply With Quote
Old April 1, 2014   #22
bower
Tomatovillian™
 
bower's Avatar
 
Join Date: Feb 2012
Location: Newfoundland, Canada
Posts: 6,794
Default

From the chart in the presentation you linked and the following I would conclude that delta carotene is on the pathway to lutein synthesis and never leading to beta carotene or lycopene. The Del gene might contribute to lutein in tomatoes, but at the expense of beta carotene or lycopene, according to the abstract for this paper; http://www.ncbi.nlm.nih.gov/pubmed/24141052
Elevation of lutein content in tomato: a biochemical tug-of-war between lycopene cyclases.

The Del variety used in that research is referred to as "HighDelta".


Foundation research on Del tomato crosses by Tomes:
http://www.genetics.org/content/62/4/769.full.pdf



I haven't seen any research on biological effects of delta carotene itself, but there's a lot of research on the health benefits of lutein and bioavailability is discussed in this abstract for example: http://www.ncbi.nlm.nih.gov/pubmed/10426704

Bioavailability of lutein from vegetables is 5 times higher than that of beta-carotene.

It's very interesting, that there are Beta 'promoters' found in different type examples. I found two articles by Francis and co-authors who are continuing to explore the additional color modifiers found in wild species, besides the classic known tomato genes/alleles.

http://journal.ashspublications.org/....full.pdf+html
Sacks and Francis (2001)

http://journal.ashspublications.org/....full.pdf+html
Kabelka Yang and Francis (2004)
bower is offline   Reply With Quote
Old April 1, 2014   #23
Darren Abbey
Tomatovillian™
 
Darren Abbey's Avatar
 
Join Date: Jun 2012
Location: Minnesota
Posts: 586
Default

Unfortunately, the "HighDelta" was one of their experimental transgenic lines and so not available for general purpose (amateur) breeding experiments.

Prolycopene is considered more bioavailable than lycopene itself, but I don't know how it relates to the more terminal carotenes.

There is some evidence out there for additional beta-carotene synthesis outside the lycopene pathway, but I haven't gathered enough information to make sense of it yet.
Darren Abbey is offline   Reply With Quote
Old April 1, 2014   #24
bower
Tomatovillian™
 
bower's Avatar
 
Join Date: Feb 2012
Location: Newfoundland, Canada
Posts: 6,794
Default

Thanks for the blog link, Darren, I missed your post while I was making mine. Your thread here has a lot of value to amateur breeders, though, so I hope you don't mind if we carry on.

I guess I shouldn't be surprised to find that Jaune Flamme has the Beta gene, since the data showed it so high in beta carotene. If JF had the tt gene, it would be prolycopene/ cis-lycopene instead, because the tt effect is dominant to the others.
bower is offline   Reply With Quote
Old April 1, 2014   #25
bower
Tomatovillian™
 
bower's Avatar
 
Join Date: Feb 2012
Location: Newfoundland, Canada
Posts: 6,794
Default

Sunray is one of the high beta carotene tomatoes identified in the TreeCropsResearch.org data. I did some searching and was very surprised to find Jubilee in the pedigree which is tt tangerine. Sunray came from a cross between Jubilee and "Pan American". But Sunray's carotene profile is not what you'd expect from tt at all.

A search for Pan American turned up more information about the pedigree, which involved introgressions from wild species, in this research article on cryptic introgressions:
http://www.biomedcentral.com/1471-2229/12/133

"Some of the earliest tomato introgression breeding in the US may have been done indirectly and unwittingly via the French variety Merville des Marchés. Recent phenotypic data collected for Merville des Marchés PI 109834 showed it to be variable in fruit size and smoothness ( http://www.ars-grin.gov/cgi-bin/npgs...lay.pl?1129442 webcite); its genotype was segregating, showed population admixture, and was an outlier based on genetic distance relative to many other S. lycopersicum accessions [8,9]. We postulated that these were indications of S. pimpinellifolium in its ancestry (this idea was examined in the current study). The Fusarium wilt-resistant processing variety Marvel [10] was selected from Merville des Marchés in the early 1900s, and Marvel was a parent of Marglobe released in 1925 [11], which in turn can be found in the pedigree of many important varieties from the 1930s through the late 1950s (H.M. Munger’s tomato pedigree chart provided by E.D. Cobb, Cornell University, 2012). Direct introgression of tomato with wild species in the US commenced in the 1930s concurrent with collection expeditions to geographic centers of origin. The first released cultivar, developed from Marglobe x S. pimpinellifolium, was aptly named Pan American [12]."

Very interesting, but not enough to conclude whether Sunray has the beta gene or some combination of promoter genes from other wild spp.
bower is offline   Reply With Quote
Old April 1, 2014   #26
Darren Abbey
Tomatovillian™
 
Darren Abbey's Avatar
 
Join Date: Jun 2012
Location: Minnesota
Posts: 586
Default

Quote:
Originally Posted by bower View Post
Thanks for the blog link, Darren, I missed your post while I was making mine. Your thread here has a lot of value to amateur breeders, though, so I hope you don't mind if we carry on.
No problem. I'm still gathering data and will continue participating in this discussion.
----

In my literature research, I've found evidence suggestive of an alternate pathway to synthesize beta-carotene. It isn't something that anyone seems to be studying directly, but it has turned up a few times as a relatively high beta-carotene in mutational contexts which should have interfered with its production by the well-studied pathway.

I wouldn't be surprised if "Sunray" was tt, but with a modifier that activated that alternate pathway. …at least this is a hypothetical situation that would be consistent.

Last edited by Darren Abbey; April 1, 2014 at 09:11 PM.
Darren Abbey is offline   Reply With Quote
Old April 2, 2014   #27
crmauch
Tomatovillian™
 
crmauch's Avatar
 
Join Date: May 2013
Location: Honey Brook, PA Zone 6b
Posts: 399
Default

I have enough 97L97 to share (not exactly sure of amount, but more than I would plant over 2 years (for a breeding plant I'm only aiming for 2 plants personally -- attempting to start 4). Darren has first dibs, but others can PM and I'll share out what I have. 5 seeds each? I'm 0 for 4 so far, just reseeded, so I can't speak for their viability.

PM me.

Chris
crmauch is offline   Reply With Quote
Old April 2, 2014   #28
bower
Tomatovillian™
 
bower's Avatar
 
Join Date: Feb 2012
Location: Newfoundland, Canada
Posts: 6,794
Default

Chris, did you pre-treat the L. cheesmanni seeds? These and others from TGRC can be difficult or impossible to germinate without treatment, although they are viable.

I did not manage to germinate some Lycopersicoides accessions last year with ordinary treatments, (Pimpinellifolium germinated but took weeks) so this year I followed the method recommended by TGRC using half strength bleach (which is pretty extreme!), and the stubborn ones are up 4/4 this time, also as quickly as tomatoes.

Check it out if you hadn't seen it: http://tgrc.ucdavis.edu/seed_germ.aspx
bower is offline   Reply With Quote
Old April 3, 2014   #29
crmauch
Tomatovillian™
 
crmauch's Avatar
 
Join Date: May 2013
Location: Honey Brook, PA Zone 6b
Posts: 399
Default

Quote:
Originally Posted by bower View Post
Chris, did you pre-treat the L. cheesmanni seeds? These and others from TGRC can be difficult or impossible to germinate without treatment, although they are viable.

I did not manage to germinate some Lycopersicoides accessions last year with ordinary treatments, (Pimpinellifolium germinated but took weeks) so this year I followed the method recommended by TGRC using half strength bleach (which is pretty extreme!), and the stubborn ones are up 4/4 this time, also as quickly as tomatoes.

Check it out if you hadn't seen it: http://tgrc.ucdavis.edu/seed_germ.aspx
I remember hearing about this, I hadn't pursued this because I was hoping since the cheesmanni was originally crossed with 4 tomatoes and then processed through to F5 that there shouldn't be any non-standard germination barriers. I replanted 97L97 yesterday. If these don't germinate, I'll look into alternate germination methods. Thanks for the link, I'll start looking at this now.
crmauch is offline   Reply With Quote
Old April 3, 2014   #30
bower
Tomatovillian™
 
bower's Avatar
 
Join Date: Feb 2012
Location: Newfoundland, Canada
Posts: 6,794
Default

Quote:
Originally Posted by crmauch View Post
I remember hearing about this, I hadn't pursued this because I was hoping since the cheesmanni was originally crossed with 4 tomatoes and then processed through to F5 that there shouldn't be any non-standard germination barriers. I replanted 97L97 yesterday. If these don't germinate, I'll look into alternate germination methods. Thanks for the link, I'll start looking at this now.
I thought the same last year, since the accession is basically a tomato with one gene from lycopersicoides. This time I did the 1/2 hour soak in 1/2 strength household bleach, rinsed thoroughly, and soaked another 1/2 hour in water to remove any residue before planting.
bower is offline   Reply With Quote
Reply


Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

Forum Jump


All times are GMT -4. The time now is 01:42 PM.


★ Tomatoville® is a registered trademark of Commerce Holdings, LLC ★ All Content ©2022 Commerce Holdings, LLC ★