My wild tomato breeding project

[FredB]

Originally Posted by Fusion_power

Most Habrochaites are susceptible to septoria to varying degrees. A few Habrochaites lines are highly resistant. The resistance often seen in rootstocks is from rampant growth together with high alkyl production. It won't hold up under heavy fruit production in a typical edible tomato genetic background. LA2175 has a high degree of genetic resistance that is transferable to domestic tomato. It just takes a lot of generations of growing plants to eliminate most of the genetic drag from Habrochaites.

Does anyone have a few seeds of LA2175 they could send me? I tried to get it from TGRC, but they said they only supply seeds to professional breeders.

[Keen101]

Today i planted seed for 4 accessions of S. galapagense, one S. cheesmaiae, 2 S. habrochaites, and 1 S. peruvianum. I used the 1:1 ratio of water:bleach method soaked for 30 min for all of them (with a little lemon juice and cream of tartar mixed in). For S. galapagense and S. cheesmaniae they actually recommend 1 hour soaking, but i only did 30 min.

My earlier pots of test S. galapagense and S. cheesmaniae seemed to have germinated well. I have at least 5 seedlings of each that i can probably separate at some point into separate pots. The S. galapagense did take longer to germinate though and when i was still unsure if they would germinate at all i did add a little lemon juice to the soil one day. Not sure if it helped, but i guess it didn't hurt.

One of the accessions i think for S. cheesmaniae for the notes i wrote on the seed packet said exerted stigma and antho, so that sounds exciting. One of the S. galapagense accessions said red fruit which is unusual. I'm hoping the orange-brown fruit one actually is brown.

[Keen101]

Ok. Update time. Just seedlings right now, but my test seedlings for this year are getting bigger and are doing well.



The Solanum cheesmaniae ones show some variation, both in terms of antho stems vs. non-antho stems, but also in smell and what might be disease/bug susceptibility. Still all very interesting though. SC LA0422



There is one tall but more yellowish plant in the S. cheesmaniae batch. This is one of the two that lack antho coloring. This is also the one that smells slightly lemony. I'll be honest in saying that the slight lemony smell slightly bugs me. This is also the plant that is showing bug and/or disease susceptibility on the leaves (might be aphids). But this one is now showing early flowering in the 6th and maybe the 4th node.. so that's interesting. I'm more interested in the antho stem ones, but i wont kill it off. I'll keep watching it. Might be interesting. The others have leaves and smell similar to domestic tomatoes.



The Solanum galapagense have very interesting leaves. I really like them! Plus something interesting is that they have no smell! Not even traditional tomato leaf smell. So that's way cool! Very interesting. Excited for these. SG LA0317





A few S. habrochaites seedlings as well. And an early Wx5 plant trimmed bonzai style with 4 tomatoes already.

[Keen101]

I guess i have officially hijacked this thread from the OP. Oh, well. Ya snooze ya loose.

Here's my update so far.

Photo 1: Solanum galapagense. I LOVE these leaves!



2. Solanum cheesmaniae



3. Solanum habrochaites



4. Misidentified as Wx5 but really Solanum cheesmaniae?



5. Regular Tomato for comparison. I believe this is the variety known as "Anasazi" that i got from Boulder, CO.

[FredB]

S. galapagense does have interesting frilly foliage. I'm surprised your galapagense variety didn't have any odor to the foliage. The four varieties I tried all had a strong odor that reminded me of burning garbage. I got my varieties from USDA GRIN, so they aren't the same as your variety from TGRC.

[Keen101]

Originally Posted by FredB

S. galapagense does have interesting frilly foliage. I'm surprised your galapagense variety didn't have any odor to the foliage. The four varieties I tried all had a strong odor that reminded me of burning garbage. I got my varieties from USDA GRIN, so they aren't the same as your variety from TGRC.

Can't remember if LA0317 was one of the ones i got from both TGRC and USDA GRIN. Regardless yeah, they do have a very light smell i guess, but barely noticeable and definitely not like burning garbage. I'd say the S. habrochaites have an off putting smell closer to that. Even in my test planting of S. cheesmaniae I've noticed variation in smells. So it's possible the same variation can exist in other wild tomato stocks, including S. galapagense.

There is one stock from the TGRC that is listed as Odorless, but it is not the accession i have, and secondly it's for S. cheesmaniae anyway. LA1018 Odorless from S. cheesmaniae

(http://tgrc.ucdavis.edu/Misc-stocks%20list%202015.pdf)

It's hard for me at this point to truly know if they are indeed Odorless or if my genetics are at play. I'm one of the people who can't stand to eat Cilantro because i taste an alkaloidal chemical that is Soapy, reminds me of stinkbugs, and makes my tongue literally go numb. People say it's because i have a recessive trait that does not allow me to smell some other chemical and so all i smell and taste is the other one underneath (which happens to actually be used in soap making). Not sure. I'm fine with Parsley though. People who love cilantro don't have that experience and think that it tastes "fresh". I once had a burrito that tasted so bad because i thought the person who made it washed their hands with soap and was just sloppy making my burrito. The soapy flavor got more and more intense that i had to just throw it on the ground and leave it there. I was so hungry that day too. made me angry.

So yeah, my smelling genetics probably affect what things i like to eat. Maybe that is why i was such a picky eater when i was young.

[Keen101]

Just wanted to update that my S. galapagense did eventually develop an odor. Not like burning garbage but noticeable. Less annoying than the Peruvianum or habrochaites smell in my opinion but not by much.

I'm excited to work with the pennellii hybrids i got from TGRC.

[Keen101]

Originally Posted by Walt

I had a class under Dr. Carl Clayberg. He was a bean breeder at that time, but he had been a noted tomato breeder. He commented in a lecture that domestic tomatoes were very hard to cross with S. peruvianum and S. Chilense, and that it had been thought for years that the F1 was just as hard to cross. Then it was discovered that unrelated F1 plants were quite interfertile.
Bear in mind that if you are working for years on crossing something with zero success that "quite fertile" might mean something like 2% fertile. So I don't know how fertile the F1 plants are.
I'm set up for tissue culture, and I've thought about trying these crosses. It is very easy to think about things. Tomato crosses are one of the easiest things in the world to think about.

Just an update. Some seedlings have emerged in the pot labeled Solanum chilense. There might be one for Solanum pennellii but it's still early, hoping for lots of little seedlings to increase the chance of at least one making it to adulthood. I don't know if i will purposely be trying to make crosses with all these wild tomatoes and hybrids and mixed-heritage lines, but i figure if they are all growing in the same garden then that increases the likely hood of any small pollinators that may visit the wild tomatoes and make natural crosses with each other hoping that some will be naturally compatible and make fruits on their own.

[Fusion_power]

self-incompatibility is the rule of thumb for wild species. Some wild species cross with each other fairly easily which can get past the S gene limits and allow fruit set.

[Keen101]

http://www.tomatoville.com/showthread.php?t=41335

http://www.tomatoville.com/showthrea...t=40417&page=4