PL trait not appearing in supposed AGG cross

scottfsmith · March 4, 2006

I saved seed from what I thought was an Aunt Gerties Gold cross that I grew out last year. The supposed cross was a RL, red-orange tomato that came from my AGG seeds. Well, I have 9 seedlings so far and none of them look PL, all look RL. That means I almost surely didn't have an AGG cross last year, because if I did I should be seeing 1/4 PL, right? I guess the odds are something like 1/5 that I just didn't grow out enough plants, but 4/5 that it was not an AGG cross that I had last year. So its looking like I just had a seed mixup? The only other thing I can think of is the tomato I saved seed from was itself pollinated by a RL, meaning only 1/16(?) would be PL. Thats also pretty unlikely...

Scott

scottfsmith · March 5, 2006

Quote:

Originally Posted by kctomato

seems to me you are answering your own questions.

I am trying to, but this is my first "genetics" experiment and even though I have read all about it I expect I may be missing something obvious. It sounds like I have it more or less right, which is good. Well, I messed up the 1/16 bit, thanks for the clarification.

One of them coming up now may be a PL.. its looking that way now but it has very small second leaves. I also planted 10 more seeds to get a bigger sample.

Scott

scottfsmith · March 8, 2006

.. we have PL! we have PL! Only one in eleven seedlings but I'll take what I can get. And to think I almost threw all of them out.

Scott

Raymondo · March 17, 2006

Interestingly, if the likelihood of seeing a trait is 1/4, as in PL in the F2 of a PL RL cross, you need to grow 11 plants to be 95% certain of seeing the PL. Growing 16 plants improves this to 99%. A likelihood of 1/4 doesn't mean 1 in four plants will be PL. You need to dig into a little statistical analysis to determine the number of plants you need to grow to give you a particular level of certainty.

March 4, 2006	#1
scottfsmith Tomatovillian™ Join Date: Feb 2006 Location: Baltimore, MD Posts: 53	PL trait not appearing in supposed AGG cross I saved seed from what I thought was an Aunt Gerties Gold cross that I grew out last year. The supposed cross was a RL, red-orange tomato that came from my AGG seeds. Well, I have 9 seedlings so far and none of them look PL, all look RL. That means I almost surely didn't have an AGG cross last year, because if I did I should be seeing 1/4 PL, right? I guess the odds are something like 1/5 that I just didn't grow out enough plants, but 4/5 that it was not an AGG cross that I had last year. So its looking like I just had a seed mixup? The only other thing I can think of is the tomato I saved seed from was itself pollinated by a RL, meaning only 1/16(?) would be PL. Thats also pretty unlikely... Scott

March 17, 2006	#4
Raymondo Tomatovillian™ Join Date: Jan 2006 Location: Saumarez Ponds, NSW, Australia Posts: 946	Interestingly, if the likelihood of seeing a trait is 1/4, as in PL in the F2 of a PL RL cross, you need to grow 11 plants to be 95% certain of seeing the PL. Growing 16 plants improves this to 99%. A likelihood of 1/4 doesn't mean 1 in four plants will be PL. You need to dig into a little statistical analysis to determine the number of plants you need to grow to give you a particular level of certainty. __________________ Ray

March 8, 2006	#3
scottfsmith Tomatovillian™ Join Date: Feb 2006 Location: Baltimore, MD Posts: 53	.. we have PL! we have PL! Only one in eleven seedlings but I'll take what I can get. And to think I almost threw all of them out. Scott